Simultaneous multi-patch-clamp and extracellular-array recordings: Single neuron reflects network activity

摘要

The increasing number of recording electrodes enhances the capability ofcapturing the network's cooperative activity, however, using too many monitorsmight alter the properties of the measured neural network and induce noise.Using a technique that merges simultaneous multi-patch-clamp andmulti-electrode array recordings of neural networks in-vitro, we show that themembrane potential of a single neuron is a reliable and super-sensitive probefor monitoring such cooperative activities and their detailed rhythms.Specifically, the membrane potential and the spiking activity of a singleneuron are either highly correlated or highly anti-correlated with thetime-dependent macroscopic activity of the entire network. This surprisingobservation also sheds light on the cooperative origin of neuronal burst incultured networks. Our findings present an alternative flexible approach to thetechnique based on a massive tiling of networks by large-scale arrays ofelectrodes to monitor their activity.